TMB Stop Reagent (TMB650)
Product Description:
Please call for pricing
TMB-HRPO ELISA Stop Solution is a propriety solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. Addition of the above STOP solution keeps the color range at 625- nm to 650 nm.
Product Stability and Storage.
Stable at 2-8 C for 3 years.
Product Use:
TMB STOP Solution(TMB650TM) is a proprietary solution used to terminate the peroxidase TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. At appropriate time, when the color intensity is appropriate, the reaction is terminated by addition of the STOP Solution. The absorbency is measured at 650 nm.
TMB HRP Aqueous Substrate 1 Component, Universal (TMBU)
Description:
Please call for pricing
TMB Ready to use HRP Aqueous Substrate 1-Component contains 3 3’5 5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a Ready to Use solution containing hydrogen peroxide. When this substrate system is reacted with peroxidase, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product, which is stable for at least one hour. By measuring the concentration of yellow product, the amount of reacting product can be detected.
Highlights:
TMB Ready to use HRP Aqueous Substrate 1-Component is stable for a minimum of 48 months from the date of manufacture when stored at 2-8 Centigrade. The substrate solution is light sensitive and should be protected from direct sunlight and UV sources.
Product Use:
TMB Ready to use HRP Aqueous Substrate 1-Component is supplied as Ready to Use solution. For ELISA Microwell applications. 50 to 100 microliters of substrate solutions is added to each well. A soluble blue color reaction product develops which can be stopped using an equal volumes of 1 N HCl, 0.18 M sulfuric acid, or Medicore Stop solution (Cat. # 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates.
TMB HRP Aqueous Substrate 1 Component, Gradual (TMBG)
Description:
Please call for pricing
This product progress slow. TMB Ready to use HRP Aqueous Substrate 1-Component contains 3 3’5 5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a Ready to Use solution containing hydrogen peroxide. When this substrate system is reacted with peroxidase, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product, which is stable for at least one hour. By measuring the concentration of yellow product, the amount of reacting product can be detected.
Highlights:
TMB Ready to use HRP Aqueous Substrate 1-Component is stable for a minimum of 48 months from the date of manufacture when stored at 2-8 Centigrade. The substrate solution is light sensitive and should be protected from direct sunlight and UV sources.
Product Use:
TMB Ready to use HRP Aqueous Substrate 1-Component is supplied as Ready to Use solution. For ELISA Microwell applications. 50 to 100 microliters of substrate solutions is added to each well. A soluble blue color reaction product develops which can be stopped using an equal volumes of 1 N HCl, 0.18 M sulfuric acid, or Medicore Stop solution (Cat. # 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates
TMB HRP Aqueous Substrate 1 Component, Enhanced (TMBE)
Description:
Please call for pricing
This product is more sensitive than universal.TMB Ready to use HRP Aqueous Substrate 1-Component contains 3 3’5 5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a Ready to Use solution containing hydrogen peroxide. When this substrate system is reacted with peroxidase, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product, which is stable for at least one hour. By measuring the concentration of yellow product, the amount of reacting product can be detected.
Highlights:
TMB Ready to use HRP Aqueous Substrate 1-Component is stable for a minimum of 48 months from the date of manufacture when stored at 2-8 Centigrade. The substrate solution is light sensitive and should be protected from direct sunlight and UV sources.
Product Use:
TMB Ready to use HRP Aqueous Substrate 1-Component is supplied as Ready to Use solution. For ELISA Microwell applications. 50 to 100 microliters of substrate solutions is added to each well. A soluble blue color reaction product develops which can be stopped using an equal volumes of 1 N HCl, 0.18 M sulfuric acid, or Medicore Stop solution (Cat. # 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates.
TMB Stop Reagent (TMB450)
Product Description:
Please call for pricing
TMB-HRPO ELISA Stop Solution is a propriety solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. At appropriate time when the color intensity is appropriate, the reaction is terminated by addition of the stop solution. The blue color turns yellow and the absorbance is measured at 450 nm within an hour.
Product Stability and Storage.
Stable at 2-8 C for 3 years.
Product Use:
After incubation with horseradish peroxidase conjugated secondary antibody (50 or 100 microliters), the wells are washed and an appropriate volume (50 or 100 microliters) of stop solution is added. The absorbency is measured at 450 nm.
TMB Stop Reagent (TMB650)
Product Description:
Please call for pricing
TMB-HRPO ELISA Stop Solution is a propriety solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. Addition of the above STOP solution keeps the color range at 625- nm to 650 nm.
Product Stability and Storage.
Stable at 2-8 C for 3 years.
Product Use:
TMB STOP Solution(TMB650TM) is a proprietary solution used to terminate the peroxidase TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. At appropriate time, when the color intensity is appropriate, the reaction is terminated by addition of the STOP Solution. The absorbency is measured at 650 nm.
Synthetic Blocking Buffer (BBS)
Product Description:
Please call for pricing
The product can be used to block nonspecific binding sites to reduce background signals. After the coating step, the Synthetic Blocking Buffer is added to the microwells for blocking the remaining free binding sites. The blocking is complete and stable.
The blocking buffer eliminates the risk of false positive reactions in the assay and at the same time avoids the hazards related to the use of material like, bovine serum albumin. The Synthetic Blocking Buffer does not contain any proteins and thus cross reactivity can be avoided.
Specialities
Available in ready-to-use aqueous formulations
No additional reagents or filtering is necessary
Product Stability:
We have tested the effectiveness in blocking and stabilization of ELISA plates. The product can be stored at 2 -8o C for four years.
Diluent Stabilizer Synthetic for HRP Conjugate (DSC)
Description
Please call for pricing
HRP in general is conjugated to IgG. In ELISA this IgG binds to specific antigen and then in presence of TMB and hydrogen peroxide, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product. By measuring the concentration of the yellow product the amount of specific antigen can be determined.
Highlights
The HRP conjugated IgG can be kept stable in a proper storage buffer. We have successfully manufactured the storage buffer which contains either a protein, BSA or a non protein component. The buffer can be stored at 4- 8 degree Centigrade. Once a HRP Conjugate solution is prepared it should be kept at 4 – 8 degree centigrade. for at least one year.-
Instructions to use
The product can be used in ELISA. An appropriate conjugate solution is prepared in Medicore Diluent Stabilizer. About 50 to 100 microliters of diluted conjugate solution are added to each microwell and incubated. After incubation , the wells were washed and 50 - 100 microliters of Medicore TMB HRP Aqueous Substrate 1-component (Catalog # 1000, 1001 or 1002) added to each well. A soluble blue color reaction product is developed which can be stopped using an equal volume of 1N HCL, 0.18 m sulfuric acid or Medicore Stop solution (Cat# 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates.
Diluent Stabilizer with BSA for HRP Conjugate (DBC)
Description
Please call for pricing
Horseradish Peroxidase (HRP) is the most commonly used enzymes in immunoassays. In the assay, HRP molecules is first conjugated to the antibody or antigen. The antibodies or antigens conjugated with HRP can be stored at 2-8°C either as a concentrated stock solution or diluted in an appropriate buffer.
When diluted biomolecules or conjugates need to be stored for future use, the solution should have stabilizing properties to maintain the molecular conformation and prevent loss of activity over time, and finally providing ready-to-use working dilutions. Medicore. Inc. offers conjugate stabilizers with stability.
Specialities:
Containing BSA. Ready-to-use. Negligible batch-to-batch variations
Stability
Protein based. The diluent contains a protein, BSA. Currently our diluents are stable for more than a year at 2 – 8 degree centigrade. We are working to lengthen the stability of these products
Primary Mouse Embryonic Fibroblasts from CD-1
Description
These are primary fibroblasts cell. Stored frozen in Freeze medium containing : 95% FBS + 5% DMSO.
We supply them as passage 1. These cells were washed, trypsinized and cryopreserved.
Propagation: DMEM (Mediatech, Inc) with 10% fetal bovine serum + 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml).
Free from potential biological contaminants
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E 12.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during cultivation. We used virus and pathogen free mice
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that may play roles in maintaining the pluripotency of ES cells.
Characteristics of MEF cells:
- These are primary cells and they have a limited life span in vitro.
- Cells grow well in most brands of tissue culture plates
- Store in liquid nitrogen until use
Primary Mouse Embryonic Fibroblasts from CF-1
Description
These are primary fibroblasts cell. Stored frozen in Freeze medium containing : 95% FBS + 5% DMSO.
We supply them as passage 1. These cells were washed, trypsinized and cryopreserved.
Propagation: DMEM (Mediatech, Inc) with 10% fetal bovine serum + 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml).
Free from potential biological contaminants
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E 12.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during cultivation. We used virus and pathogen free mice
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that may play roles in maintaining the pluripotency of ES cells.
Characteristics of MEF cells:
- These are primary cells and they have a limited life span in vitro.
- Cells grow well in most brands of tissue culture plates
- Store in liquid nitrogen until use
Primary Mouse Embryonic Fibroblasts from Transgenic Mice containing neomycin resistant gene
Description
These are primary fibroblasts cell. Stored frozen in Freeze medium containing : 95% FBS + 5% DMSO.
We supply them as passage 1. These cells were washed, trypsinized and cryopreserved.
Propagation: DMEM (Mediatech, Inc) with 10% fetal bovine serum + 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml).
Free from potential biological contaminants
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E 13.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during cultivation. We used virus and pathogen free mice
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that may play roles in maintaining the pluripotency of ES cells.
Characteristics of MEF cells:
- These are primary cells and they have a limited life span in vitro.
- Carry neomycin resistance markers allowing selection under neomycin
- Cells grow well in most brands of tissue culture plates
- Store in liquid nitrogen until use
Primary Mouse Embryonic Fibroblasts from Transgenic Mice containing hygromycin resistant gene
Description
These are primary fibroblasts cell. Stored frozen in Freeze medium containing : 95% FBS + 5% DMSO.
We supply them as passage 1. These cells were washed, trypsinized and cryopreserved.
Propagation: DMEM (Mediatech, Inc) with 10% fetal bovine serum + 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml).
Free from potential biological contaminants
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E 13.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during cultivation. We used virus and pathogen free mice
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that may play roles in maintaining the pluripotency of ES cells.
Characteristics of MEF cells:
- These are primary cells and they have a limited life span in vitro.
- Carry hygromycin resistance markers allowing selection under hygromycin
- Cells grow well in most brands of tissue culture plates
- Store in liquid nitrogen until use
