Mouse Ventral Mesencephalon E14 Neurospheres (Neural Stem cells)
Freeze medium: 90% “Complete Proliferation medium StemCell Technologies)+ 10% DMSO.
Medium renewal: 2 to 3 times weekly.
Growth properties: Suspension or adherent culture depending on medium (see below).
Comments: These cells are primary cells. We supply them as passage 1. These cells can be expanded to passage 3 to 6 without negligible loss of integrity.
Proliferation/ Propagation: Basal Medium (StemCell Technologies, Cat# 05700) supplemented with Proliferation Supplements (StemCell Technologies, Cat# 05701), human Epidermal Growth Factor, rh EGF and 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml)
Differentiation. Basal Medium, supplemented with Differentiation Supplements.
The Functional Assays. The Functional Assays employed were (i) Stem cell Proliferation Assays using either a monolayer system or a neurosphere system and showed high expression of Nestin and SOX2 and (ii) Multipotential differentiation assays into Astrocytes, Neurons and Oligodendrocytes through marker analysis..
Primary Embryonic Neurospheres are isolated from CD 1 mouse embryos at E 14 day. These are primary cells and as such have a limited life span in culture. All cryopreserved neurospheres contain neural stem cells and progenitor cells. Cultured stem cells represent a potential cell source for transplantation and useful model for drug development, for studies of neurotoxicity, neurogenesis, neurotransmitter and CNS diseases and disorders. A healthy state of culture is maintained during cultivation. We used virus and pathogen free animals. We have used our 25+ years of tissue culture experience in preparation of these primary cells.
Shipped: Frozen, store in liquid nitrogen until use.
Other Characteristics of Neurospheres:
- These are primary cells and they have a limited life span in vitro.
- Cells grow well in most brands of tissue culture plates