Rat Spinal Cord E18 Neurospheres (Neural Stem cells)
Freeze medium: 90% “Complete” Proliferation medium (available from StemCell Technologies or other sources)+ 10% DMSO.
Medium renewal: 2 to 3 times weekly.
Growth properties: Suspension or adherent culture depending on medium (see below).
Comments: These cells are primary cells. We supply them as passage 1.
Proliferation/ Propagation: Basal Medium (StemCell Technologies, Cat# 05770) supplemented with Proliferation Supplements (StemCell Technologies, Cat# 05773), human Epidermal Growth Factor, rh EGF , human Fibroblast Growth Factor-basic, heparin (StemCell Technologies, Cat# 07980) and 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml). Details:
Differentiation. Basal Medium, supplemented with Differentiation Supplements. For details see Technical Manual.
The Functional Assays. The Functional Assays employed were (i) Stem cell Proliferation Assays using either a monolayer system or a neurosphere system and showed high expression of Nestin and SOX2 and Multipotential differentiation assays into Astrocytes, Neurons and Oligodendrocytes through marker analysis..
Primary Embryonic Neurospheres are isolated from Sprague-Dawley rat embryo cortex at E 18 day. These are primary cells and as such have a limited life span in culture. All cryopreserved neurospheres contain neural stem cells and progenitor cells. Cultured stem cells represent a potential cell source for transplantation and useful model for drug development, for studies of neurotoxicity, neurogenesis, neurotransmitter and CNS diseases and disorders. A healthy state of culture is maintained during cultivation. We used virus and pathogen free animals
Shipped: Frozen, store in liquid nitrogen until use.
Other Characteristics of Neurospheres:
- These are primary cells and they have a limited life span in vitro.
- Cells grow well in most brands of tissue culture plates