• Home
  • Products
  • Services
  • About us
  • Contact
MEDICORE, INC
  • Home
  • Products
  • Services
  • About us
  • Contact
  • Neural Stem Cells
  • >
  • Rat Spinal Cord E18 Neurospheres (Neural Stem cells)

Rat Spinal Cord E18 Neurospheres (Neural Stem cells)

$495.00
$495.00
Unavailable
per item

Description

Morphology: Spheroid.

Freeze medium: 90% “Complete” Proliferation medium (available from StemCell Technologies or other sources)+ 10% DMSO.

Medium renewal: 2 to 3 times weekly.

Growth properties: Suspension or adherent culture depending on medium (see below).

Comments: These cells are primary cells.  We supply them as passage 1.

Proliferation/ Propagation:  Basal Medium (StemCell Technologies, Cat# 05770) supplemented with   Proliferation Supplements (StemCell Technologies, Cat# 05773), human Epidermal Growth Factor, rh EGF ,  human Fibroblast Growth Factor-basic, heparin (StemCell Technologies, Cat# 07980) and 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml). Details:

Differentiation. Basal Medium, supplemented with Differentiation Supplements. For details see Technical Manual.

The Functional Assays. The Functional Assays employed were (i) Stem cell Proliferation Assays using either a monolayer system or a neurosphere system and showed high expression of Nestin and SOX2  and Multipotential differentiation assays into Astrocytes, Neurons and Oligodendrocytes through marker analysis..


Primary Embryonic Neurospheres are isolated from Sprague-Dawley rat embryo cortex at E 18 day.  These are primary cells and as such have a limited life span in culture. All cryopreserved neurospheres contain neural stem cells and progenitor cells. Cultured stem cells represent a potential cell source for transplantation and useful model for drug development, for studies of neurotoxicity, neurogenesis, neurotransmitter and CNS diseases and disorders.  A healthy state of culture is maintained during cultivation. We used virus and pathogen free animals

Shipped: Frozen, store in liquid nitrogen until use.


Other Characteristics of Neurospheres:

  • These are primary cells and they have a limited life span in vitro.
  • Cells grow well in most brands of tissue culture plates



SKU: R-SpN
  • Facebook
  • Twitter
  • Pinterest
  • Google+
Add to Cart

Home

Products

Services

About us

Contact


Picture
Medicore Inc
13 Taft Court, Suite 230, Rockville, MD 20850      
​
Tel: 301.279.7979        Contact: medicore@usa.net
  • Home
  • Products
  • Services
  • About us
  • Contact