Primary Mouse Embryonic Fibroblasts from Transgenic Mice containing puromycin resistant gene
These are primary fibroblasts cell. Stored frozen in Freeze medium containing : 95% FBS + 5% DMSO.
We supply them as passage 2. These cells were washed, trypsinized and cryopreserved.
Propagation: DMEM (Mediatech, Inc) with 10% fetal bovine serum + 1% penicillin-Streptomycin (5,000 U/ml - 5,000 micrograms/ml).
Free from potential biological contaminants
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E 13.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during cultivation. We used virus and pathogen free mice
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that may play roles in maintaining the pluripotency of ES cells.
Characteristics of MEF cells:
- These are primary cells and they have a limited life span in vitro.
- Carry puromycin resistance markers allowing selection under puromycin
- Cells grow well in most brands of tissue culture plates
- Store in liquid nitrogen until use