TMB HRP Aqueous Substrate 1 Component, Universal (TMBU)
Description:
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TMB Ready to use HRP Aqueous Substrate 1-Component contains 3 3’5 5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a Ready to Use solution containing hydrogen peroxide. When this substrate system is reacted with peroxidase, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product, which is stable for at least one hour. By measuring the concentration of yellow product, the amount of reacting product can be detected.
Highlights:
TMB Ready to use HRP Aqueous Substrate 1-Component is stable for a minimum of 48 months from the date of manufacture when stored at 2-8 Centigrade. The substrate solution is light sensitive and should be protected from direct sunlight and UV sources.
Product Use:
TMB Ready to use HRP Aqueous Substrate 1-Component is supplied as Ready to Use solution. For ELISA Microwell applications. 50 to 100 microliters of substrate solutions is added to each well. A soluble blue color reaction product develops which can be stopped using an equal volumes of 1 N HCl, 0.18 M sulfuric acid, or Medicore Stop solution (Cat. # 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates.
