TMB HRP Aqueous Substrate 1 Component, Universal (TMBU)
TMB Ready to use HRP Aqueous Substrate 1-Component contains 3 3’5 5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a Ready to Use solution containing hydrogen peroxide. When this substrate system is reacted with peroxidase, a soluble blue reaction product is produced. The reaction can be stopped using appropriate stop solution producing a soluble yellow reaction product, which is stable for at least one hour. By measuring the concentration of yellow product, the amount of reacting product can be detected.
TMB Ready to use HRP Aqueous Substrate 1-Component is stable for a minimum of 48 months from the date of manufacture when stored at 2-8 Centigrade. The substrate solution is light sensitive and should be protected from direct sunlight and UV sources.
TMB Ready to use HRP Aqueous Substrate 1-Component is supplied as Ready to Use solution. For ELISA Microwell applications. 50 to 100 microliters of substrate solutions is added to each well. A soluble blue color reaction product develops which can be stopped using an equal volumes of 1 N HCl, 0.18 M sulfuric acid, or Medicore Stop solution (Cat. # 2000). The blue color is changed to yellow color and can be read at 450 nm. The intensity of the color can be controlled using diluted antibodies or conjugates.